千葉由佳子 准教授 / CHIBA, Yukako

RNAから迫る植物の環境適応機構と生存戦略

千葉由佳子 准教授 /  CHIBA, Yukako

研究分野・テーマ・内容

研究分野 植物分子生物学
研究テーマ RNAから迫る植物の環境応答機構と生存戦略

研究内容

植物は様々な環境変化の下で生育しており,移動という手段により回避することができない分,それらに迅速かつ巧妙に対処しなければなりません。これには様々な遺伝子の発現調節を伴いますが,これまでの研究のほとんどはmRNA合成の段階である転写制御に注目して行われてきました。しかしながら,実際の細胞内のmRNA量は合成と分解のバランスにより調節されています。我々はmRNA合成と分解の両方の制御機構を理解することによって,遺伝子発現における植物の持つ巧妙な環境応答機構を明らかにすることを目指しています。

メッセージ

私はDNAに刻まれた遺伝情報の発現がどのように制御されて,多彩な生命現象を支えているのかを明らかにしたいと思い研究を始めました。我々の研究室では,分子遺伝学・生理生化学的研究などの多彩な手法が可能な植物を研究対象とし,特に転写後調節に着目した分子レベルの研究を進めています。目には見えないミクロの世界の研究ですが,植物の環境応答機構の研究は将来的には過酷な環境に耐えうる食物の作成にもつながり得るものであると考えています。

参考文献・論文・著書

  • Chiba, Y., Mineta, K., Hirai, Y. M., Suzuki, Y., Kanaya, S., Takahashi, H., Onouchi, H., Yamaguchi, J., and Naito, S. Changes in mRNA stability associated with cold stress in Arabidopsis cells. Plant Cell Physiol., 54: 180-194 (2013)
  • Tanaka, M., Takano, J., Chiba, Y., Lombardo, F., Ogasawara, Y., Onouchi, H., Naito, S. and Fujiwara, T.: Boron-Dependent Degradation of NIP5;1 mRNA for Acclimation to Excess Boron Conditions in Arabidopsis. Plant Cell,23: 3547-3559 (2011)
  • Chiba, Y. and Green, P.J.: mRNA degradation machinery in plants. J. Plant Biol., 52: 114-124 (2009)
  • Chiba, Y., Johnson, M.A., Lidder, P., Vogel, J.T., Van Erp, H., Green, P.J: AtPARN is an essential poly(A) ribonuclease in Arabidopsis. Gene, 328: 95-102. (2004)
  • Chiba, Y., Ishikawa, M., Kijima, F., Tyson, R.H., Kim, J., Yamamoto, A., Nambara, E., Leustek, T., Wallsgrove, R.M. and Naito, S: Evidence for autoregulation of cystathionine γ-synthase mRNA stability in Arabidopsis. Science, 286: 1371-1374 (1999)

nameCHIBA Yukako

Research subject

Specialized field

Plant Molecular Biology

Key words

・RNA
・Stress response
・Arabidopsis

Research subject

Control of gene expression is exerted by multiple steps such as transcription, RNA processing and export, mRNA stability, translation and post-translational events. Recent discovery of small RNAs has enhanced the impact of post-transcriptional regulation, in particular alterations of mRNA stability on gene regulations. Control of mRNA turnover is important not only for changing the level of mRNA, but also for determining the transition time of mRNA, which is critical for quick response to environmental changes. The goal of our projects is to demonstrate the importance of mRNA turnover control in the regulating gene expression in response to stress conditions in plants.

message

Research in our lab focuses on mRNA turnover control of the gene expression in response to stress in higher plants. According to the kinetics of mRNA turnover, regulation of mRNA stability has a pivotal role to adjust the mRNA level to environmental changes; however the significance of the regulation is poorly understood. We are trying to understand the molecular mechanism of mRNA stability control in response to stress by using genetic and biochemical approaches.

references

  • Chiba, Y., Mineta, K., Hirai, Y. M., Suzuki, Y., Kanaya, S., Takahashi, H., Onouchi, H., Yamaguchi, J., and Naito, S. Changes in mRNA stability associated with cold stress in Arabidopsis cells. Plant Cell Physiol., 54: 180-194 (2013)
  • Tanaka, M., Takano, J., Chiba, Y., Lombardo, F., Ogasawara, Y., Onouchi, H., Naito, S. and Fujiwara, T.: Boron-Dependent Degradation of NIP5;1 mRNA for Acclimation to Excess Boron Conditions in Arabidopsis. Plant Cell,23: 3547-3559 (2011)
  • Chiba, Y. and Green, P.J.: mRNA degradation machinery in plants. J. Plant Biol., 52: 114-124 (2009)
  • Chiba, Y., Johnson, M.A., Lidder, P., Vogel, J.T., Van Erp, H., Green, P.J: AtPARN is an essential poly(A) ribonuclease in Arabidopsis. Gene, 328: 95-102. (2004)
  • Chiba, Y., Ishikawa, M., Kijima, F., Tyson, R.H., Kim, J., Yamamoto, A., Nambara, E., Leustek, T., Wallsgrove, R.M. and Naito, S: Evidence for autoregulation of cystathionine γ-synthase mRNA stability in Arabidopsis. Science, 286: 1371-1374 (1999)