ケビン・ウェイクマン 助教 / WAKEMAN, Kevin

海へ行って未知の「進化」を発見しよう

ケビン・ウェイクマン 助教 /  WAKEMAN, Kevin

研究分野・テーマ・内容

研究分野 生物多様性・進化学
研究テーマ 海産アピコンプレクサの多様性と進化;底生性渦鞭毛藻の多様性と毒性の解明

研究内容

広く原生生物(単細胞真核生物)と呼ばれる生物の多様性と進化について研究しています。現在までに、カナダ近辺の東太平洋や日本周辺の西太平洋(沖縄・東京・北海道)に住む海産生物を調べてきました。

私が最も関心をもってきたのは、無脊椎動物に寄生するアピコンプレクサ類です。主に研究しているアピコンプレクサのグループはグレガリナと呼ばれ、アピコンプレクサの中で最も原始的なグループか、あるいはその一つと考えられています。彼らは多毛類(ゴカイなど)、甲殻類(カニやエビなど)、ホヤ類などの無脊椎動物へ寄生します。海産アピコンプレクサの他の系統には、サンゴや二枚貝などに寄生するものもいます。このような海産アピコンプレクサの多様性およびアピコンプレクサ同士や宿主との関係性への知識を得ることによって、本グループに対する進化の見方を一変させたり高めていくことが私の目標です。

!cid_7E4BC98F-63C3-46D6-8ABD-76E9ECD86C6B

海産無脊椎動物の寄生生物グレガリナ類

近年は底生性渦鞭毛藻(微細藻類)の多様性と毒生産についても調べています。特に亜熱帯の沖縄に分布する渦鞭毛藻に興味を持っています。本研究には微細藻類の大量培養と高速液体クロマトグラフィー質量分析(HPLC-MS)のような化学的要素が関わってきます。

CooliaJSPS

有毒渦鞭毛藻Cooliaの一種

私の研究では、分子系統学、各種顕微鏡法、生化学、生物の培養等の幅広い手法や技術を使います。また、SCUBAダイビング/シュノーケリングを使い比較的浅瀬から採集した生物だけでなく、時として潜水艇を使ってのはるか深海からの生物までをも調べています。

!cid_8B9850FB-E0A0-42BB-A4C0-4010D80D07A1

スキューバによるサンプル採集

メッセージ

私の研究に興味をもってくれることを歓迎します。様々なバックグラウンド/興味(例:遺伝学、化学、SCUBAダイビング、顕微鏡観察など)をもつ学生が意欲をもって取り組める研究テーマを見つけられると思います。私自身は自然の中に見られる生物の多様性、特に海に住む生物達に惹き付けられています。また、学生諸君へは、海を探索することに多くの時間を費やし、‘自分の物’と言えるような何かを見つけることを勧めます。

参考文献・論文・著書

・Wakeman, K.C., Yamaguchi, A., Roy, M.C. and Jenke-Kodama, H. (2015) Morphology, phylogeny and novel chemical compounds from C. malayensis (Dinophyceae) from Okinawa, Japan. Harmful Algae. 44:8-19.

・Rueckert, S., Wakeman, K.C., Jenke-Kodama, H. and Leander, B.S. (2015) Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five new species of Lankesteria. Int. J. Syst. Evol. Biol. 65:2598-2614.

・Wakeman, K.C., Heintzelman, M.B. and Leander, B.S. (2014) Comparative ultrastructure and molecular phylogeny of two sister species, Selenidium melongena nov. sp. and Selenidium terebellae Ray 1930 demonstrate niche partitioning in marine gregarine parasites. Protist. 165:493-511.

・Wakeman, K.C., Reimer, J.D., Jenke-Kodama, H. and Leander, B.S. (2014) Molecular phylogeny and ultrastructure of Caliculium glossobalani n. gen. et sp. (Apicomplexa) from a Pacific Glossobalanus minutus (hemichordata) confounds the relationships between marine and terrestrial gregarines. J. Eukaryot. Microbiol. 61:343-353.

・Wakeman, K.C. and Leander, B.S. (2013) Molecular phylogeny of marine gregarine parasites (Apicomplexa) from tube-forming polychaetes (Sabellariidae, Cirratulidae and Serpulidae), including descriptions of four new species of Selenidium. J. Eukaryot. Microbiol. 43:133-147.

・Wakeman, K.C. and Leander, B.S. (2013) Identity of environmental DNA sequences using descriptions of four novel marine gregarine parasites, Polyplicarium n. gen. (Apicomplexa), from capitellids polychaetes. Marine Biodiversity. 43:133-147.

・Rueckert, S., Wakeman, K.C. and Leander B.S. (2013) Discovery of a diverse clade of gregarine apicomplexans from Pacific eunicid polychaetes, including descriptions of Paralecudina gen. n., Trichotokara japonica sp. n., and T. eunicae sp. n. J. Eukaryot. Microbiol. 60:121-136.

・Wakeman, K.C. and Leander, B.S. (2012) Molecular phylogeny of Pacific archigregarines (Apicomplexa), including descriptions of Velloxidium leptosynaptae n. gen. et sp. from the sea cucumber Leptosynapta clarki (Echinodermata) and two new species of Selenidium. J. Eukaryot. Microbiol. 59:232-245.

nameKevin Wakeman

Research subject

Specialized field

Marine apicomplexan and dinoflagellate (alveolate) diversity and evolution

Key words

・Protistology
・Biodiversity; Microscopy
・Genetics
・Phylogenomics
・Transcriptomics
・Toxicity
・Symbiosis

Research subject

I study the diversity and evolution of a broad range of organisms, generally referred to as protists (single-celled eukaryotes). Up to this point, I have primarily studied organisms living in marine environments, in areas of the Eastern Pacific Ocean, near Canada, as well as the Western Pacific waters around Japan (Okinawa, Tokyo, and Hokkaido).

My interests have mostly revolved around apicomplexan parasites of invertebrates. The particular assemblage of apicomplexan parasites I study are referred to as gregarines; they are some of the earliest lineages, if not the earliest lineage, of apicomplexans. They parasitize invertebrates such as polychaetes (marine worms), crustaceans (e.g., crabs and shrimp), and tunicates (just to name a few). Other lineages of marine apicomplexans also infect invertebrate groups such as coral and mussels (shellfish). Overall, by improving our knowledge of the biodiversity of marine apicomplexans, and investigating the relationships they have with each other and their host groups, it is my goal to fundamentally transform and enhance how evolution is viewed within this group.

!cid_7E4BC98F-63C3-46D6-8ABD-76E9ECD86C6B

Gregarine parasites of marine worms

More recently, I have started to study benthic marine dinoflagellates (microalgae), their diversity and production of toxin compounds. I am particularly interested in the diversity of dinoflagellates in warmer waters, especially those around the subtropical island of Okinawa, Japan. This work involves the mass cultivation of microalgae, and has some chemical elements such as High-pressure liquid chromatography-mass spectrometry (HPLC-MS).

CooliaJSPS

A species of Coolia, a toxic dinoflagellate

My research employs a wide-range of tools and techniques including molecular phylogenetics and microscopy, biochemistry, and the cultivation of organisms. This research examines organisms from relative shallow water by SCUBA diving/snorkeling, all the way to the deep sea, for which exotic deep-sea submersibles are used.

!cid_8B9850FB-E0A0-42BB-A4C0-4010D80D07A1

SCUBA diving while sampling

message

Students are always welcome and encouraged to participate in this research. I think students from various backgrounds/interests will have no problem finding a theme that inspires them (e.g., genetics, chemistry, SCUBA diving, microscopy etc.). I am personally intrigued by the diversity exhibited in nature, especially those organisms living in the ocean. And so, I encourage students to spend lots of time exploring the marine environment to find something that they can uniquely ‘own’.

references

・Wakeman, K.C., Yamaguchi, A., Roy, M.C. and Jenke-Kodama, H. (2015) Morphology, phylogeny and novel chemical compounds from C. malayensis (Dinophyceae) from Okinawa, Japan. Harmful Algae. 44:8-19.

・Rueckert, S., Wakeman, K.C., Jenke-Kodama, H. and Leander, B.S. (2015) Molecular systematics of marine gregarine apicomplexans from Pacific tunicates, with descriptions of five new species of Lankesteria. Int. J. Syst. Evol. Biol. 65:2598-2614.

・Wakeman, K.C., Heintzelman, M.B. and Leander, B.S. (2014) Comparative ultrastructure and molecular phylogeny of two sister species, Selenidium melongena nov. sp. and Selenidium terebellae Ray 1930 demonstrate niche partitioning in marine gregarine parasites. Protist. 165:493-511.

・Wakeman, K.C., Reimer, J.D., Jenke-Kodama, H. and Leander, B.S. (2014) Molecular phylogeny and ultrastructure of Caliculium glossobalani n. gen. et sp. (Apicomplexa) from a Pacific Glossobalanus minutus (hemichordata) confounds the relationships between marine and terrestrial gregarines. J. Eukaryot. Microbiol. 61:343-353.

・Wakeman, K.C. and Leander, B.S. (2013) Molecular phylogeny of marine gregarine parasites (Apicomplexa) from tube-forming polychaetes (Sabellariidae, Cirratulidae and Serpulidae), including descriptions of four new species of Selenidium. J. Eukaryot. Microbiol. 43:133-147.

・Wakeman, K.C. and Leander, B.S. (2013) Identity of environmental DNA sequences using descriptions of four novel marine gregarine parasites, Polyplicarium n. gen. (Apicomplexa), from capitellids polychaetes. Marine Biodiversity. 43:133-147.

・Rueckert, S., Wakeman, K.C. and Leander B.S. (2013) Discovery of a diverse clade of gregarine apicomplexans from Pacific eunicid polychaetes, including descriptions of Paralecudina gen. n., Trichotokara japonica sp. n., and T. eunicae sp. n. J. Eukaryot. Microbiol. 60:121-136.

・Wakeman, K.C. and Leander, B.S. (2012) Molecular phylogeny of Pacific archigregarines (Apicomplexa), including descriptions of Velloxidium leptosynaptae n. gen. et sp. from the sea cucumber Leptosynapta clarki (Echinodermata) and two new species of Selenidium. J. Eukaryot. Microbiol. 59:232-245.