Report of Systematic Zoology Lab Practicum, August, 2012
Laboratory Course in Systematic Zoology 2012
Hiroshi Kajihara
Introduction Notocomplana humilis (Stimpson, 1857): 28S large subunit ribosomal RNA gene partial sequence of Notocomplana humilis (Platyhelminthes: Rhabditophora: Polycladida: Leptoplanoidea: Notoplanidae) by Hiroki Kawamoto and Ippei Kojima. Baseodiscus sp.: 28S rDNA partial sequence of Baseodiscus sp. (Nemertea: Heteronemertea) by Takahiro Yamashita and Matasaburo Fukutomi. Tetrastemma pseudocoronatum Chernyshev, 1998: 28S ribosomal RNA, partial sequence of Tetrastemma pseudocoronatum (Nemertea: Hoplonemertea) by Takuma Nakamura and Yuta Nakamura. Oerstedia phoresiae (Kurikova, 1987): 28S rDNA partial sequence of Oerstedia phoresiae (Nemertea: Hoplonemerta: Monostilifera) by Toshihiro Arae and Mayu Horie. Polydora sp.: 28S rRNA partial sequence of Polydora sp. (Annelida: Spionidae), boring the shell of Chlorostoma turbinatum Adams, 1853 (Gastropoda: Trochidae) by Minako Ito and Hikaru Osajima. Sylliidae gen. et sp. indet.: Observation of a sylliid polychaete (Annelida: Sylliidae) by Takahiro Kato and Hiroki Ebina. Phascolosoma scolops (Selenka and De Man, 1883): Cytochrome c oxidase subunit I partial sequence of Phascolosoma scolops (Sipuncula) by Yuri Tanaka and Akari Toyoda. Flabellina athadona (Bergh, 1875): Cytochrome c oxidase subunit I partial sequence of Flabellina athadona (Bergh, 1875) (Opisthobranchia: Aeolidoidea) by Azusa Yoshida. Cleantiella isopus (Miers, 1881): Cytochrome c oxidase submit I partial sequence of Cleantiella isopus (Crustacea: Isopoda: Valvifera) by Tomofumi Otake and Keisuke Kozima. Dynoides dentisinus Shen, 1929: 28S rDNA partial sequence of Dynoides dentisinus (Crustacea: Isopoda: Sphaeromatidae) by Atsushi Saito. Leptochelia itoi Ishimaru, 1985: 28S rDNA partial sequence of Leptochelia itoi (Crustacea: Tanaidacea) by Shinya Kawata and Akari Kanda. Trachysalambria curvirostris (Stimpson, 1860): Cytochrome c oxidase subunit I partial sequence of Trachysalambria curvirostris (Crustacea: Decapoda: Penaeidae) by Ikumasa Ganaha and Ryo Mizuyama. Pagurus minutus Hess, 1865: 28S rDNA partial sequence of Pagurus minutus (Decapoda: Anomura: Paguridae) by Misato Seki and Syunsuke Togawa. Paradorippe granulata (De Haan, 1841) (De Haan, 1833): Partial sequence of cytochrome c oxidase subunit I gene from Paradorippe granulata (Crustacea: Decapoda) by Kentaro Yamada and Kousuke Watanabe. Amphipholis kochii Lütken, 1872: 28S rDNA partial sequence of Amphipholis kochii (Echinodermata: Ophiuroidea) by Mizuho Kino and Chihiro Tobita. Echinocardium cordatum Pennant, 1777: 28S ribosomal RNA gene partial sequence of Echinocardium cordatum (Echinodermata: Echinoidea: Spatangoida) by Yuki Sasaki and Kouki Murano.
Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
In Systematic Zoology Lab Practicum 2012, 30 undergraduate students in the Division of Biology, Department of Biological Sciences, School of Science, Hokkaido University went to Oshoro Marine Biological Station on 21 May, 28 May, 4 June, and 25 June 2012 to collect marine invertebrates, particularly among laminarian holdfasts and mussel beds. Clumps of the laminarian holdfasts and mussels were dissected at the marine station, and various invertebrates that crawled out from the sediments were sorted out and roughly identified. Each pair of students selected one invertebrate specimen, which was photographed in living state and fixed in 99% EtOH.
After bringing the specimens back to the laboratory in the Sapporo campus, the students extracted DNA, amplified mitochondrial cytochrome c oxidase subunit I gene (COI), and determined the sequence. In some cases they were not able to amplify COI region; in such cases they sequenced 28S rDNA instead.
Students' reports
Phylum Platyhelminthes
Phylum Nemertea
Phylum Annelida
Phylum Mollusca
Phylum Arthropoda
Phylum Echinodermata