Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Introduction
In Systematic Zoology Lab Practicum 2013, 39 third-year undergraduate students in the Division of Biology, Department of Biological Sciences, School of Science, Hokkaido University, visited Oshoro Marine Biological Station on 20 May, 27 May, 3 June, and 12 June 2013. They collected marine invertebrates among laminarian holdfasts and mussel beds. Each pair of students selected one invertebrate specimen, which was photographed in living state and fixed in 99% EtOH.
Fig. 1. Walking from the Oshoro Bus Stop along a hill that leads to the marine station, 20 May 2013, photo by Takumi Onishi.
Fig. 2. Arriving at the marine station, 3 June 2013, photo by Takumi Onishi.
Fig. 3. Wading along the rocky shore, 27 May 2013.
In the Sapporo campus, the students extracted DNA from their material, tried to PCR-amplify the mitochondrial cytochrome c oxidase subunit I gene (COI), and determined the sequence. In cases where COI amplification failed, they tired 16S and/or 28S rDNA instead. Twenty species in five phyla are reported here: Cnidaria, 1 sp.; Annelida 1 sp.; Mollusca 10 spp.; Arthropoda 5 spp.; and Echinodermata 3 spp.
Students' reports
Phylum Cnidaria
Anthopleura fuscoviridis Carlgren, 1949: 28S large subunit ribosomal RNA gene partial sequence of Anthopleura fuscoviridis (Cnidaria: Hexacorallia: Actiniaria) from Oshoro Bay, Hokkaido, Japan by Reo Uno and Shino Sugawara.
Phylum Annelida
Hydroides ezoensis Okuda, 1934: 28S rDNA partial sequence of Hydroides ezoensis (Annelida: Serpulidae) from Oshoro, Hokkaido, Japan by Kensuke Saitou and Motoki Koizumi.
Phylum Mollusca
Lepidozona coreanica (Reeve, 1847): Cytochrome c oxidase subunit I partial sequence of the chiton Lepidozona coreanica (Mollusca: Polyplacophora: Neoloricata) from Oshoro, Hokkaido, northern Japan by Satoko Mori and Kusuko Obara.
Mytilus galloprovincialis Lamarck, 1819: Cytochrome c oxidase subunit I gene partial sequence of the Mediterranean mussel Mytilus galloprovincialis (Mollusca: Bivalvia) from Oshoro Bay, Hokkaido, northern Japan by Masaki Miyajima and Akane Ito.
Crassostrea gigas (Thunberg, 1793): Cytochrome c oxidase subunit I partial sequence of Crassostrea gigas (Mollusca: Bivalvia: Ostreidae) by Yuuki Kawagishi and Mio Sato.
Chlamys (Azumapecten) farreri akazara (Kuroda, 1932): 28S ribosomal DNA partial sequence of the scallop Chlamys (Azumapecten) farreri akazara (Mollusca: Bivalvia) from Oshoro Bay, Hokkaido, Japan by Takumi Adachi and Wataru Saitou.
Chlorostoma lischkei (Tapparone-Canefri, 1874): A partial sequence of the mitochondrial cytochrome c oxidase subunit I gene in the sea snail Chlorostoma lischkei (Mollusca: Gastripoda: Trochidae) from Oshoro Bay, Hokkaido, northern Japan by Akiho Shibata and Kaito Mikuni.
Omphalius rusticus (Gmelin, 1791): Partial sequence of the nuclear 28S rRNA gene of the sea snail Omphalius rusticus (Mollusca: Gastropoda: Trochidae) from the intertidal zone in Oshoro Bay on the Sea-of-Japan coast of Hokkaido, northern Japan by Yuriko Saheki and Kenta Tahara.
Monodonta confusa Tapparone-Canefri, 1874: 28S large subunit ribosomal RNA gene partial sequence of Monodonta confusa (Mollusca: Gastropoda: Vetigastropoda: Trochidae) by Mitsuhashi Kei and Katata Satomi.
Nucella freycinetii (Deshayes, 1839): Cytochrome c oxidase subunit I partial sequence of Nucella freycinetii (Mollusca: Gastropoda: Muricoidea) from Oshoro, Hokkaido, Japan by Kango Tsubouchi and Hitoshi Nunome
Vayssierea elegans (Baba, 1930): An attempt to sequence the cytochrome c oxidase subunit I gene from Vayssierea elegans (Mollusca: Gastropoda: Nudibranchia) by Yui Kato.
Aeolidia papillosa (Linnaeus, 1761): Cytochrome c oxidase subunit I gene partial sequence of Aeolidia papillosa (Linnaeus, 1761) (Mollusca: Gastropoda: Nudibranchia) by Madoka Asano and Kohei Oguchi.
Phylum Arthropoda
cf. Caprella scaura (Templeton, 1836): Cytochrome c oxidase subunit I partial sequence of cf. Caprella scaura (Arthropoda: Crustacea: Caprellidae) from Oshoro, Hokkaido, Japan by Takuya Teranishi and Haruka Okamura.
Ptilohyale barbicornis (Hiwatari and Kajihara, 1981): 28S rDNA partial sequence of the gammaridean amphipod Ptilohyale barbicornis (Arthropoda: Crustacea) from Oshoro Bay, Hokkaido, Japan by Koutarou Tamura and Azusa Nakamoto.
Ligia cinerascens Budde-Lund, 1885: Partial sequence of the mitochondrial cytochrome c oxidase subunit I gene from putative, unidentified mites associated with the sea slater Ligia cirerascens (Arthropoda: Crustacea: Isopoda) collected in Oshoro Bay, Hokkaido, Japan by Makiko Take and Yoko Hasegawa.
Paguristes ortmanni Miyake, 1978: An attemt to PCR-amplify the 28S large subunit ribosomal RNA gene and the cytochromec oxidase subunit I in the hermit crab Paguristes ortmanni (Crustacea: Anomura: Diogenidae) collected in Oshoro Bay, Hokkaido, Japan by Shuhei Kawabata and Kazuya Kito.
Glebocarcinus amphioetus (Ruthbun, 1898): Partial sequence of the mitochondrial cytochrome c oxidase subunit I gene in the crab Glebocarcinus amphioetus (Decapoda: Brachyura: Cancridae) from Oshoro Bay, Hokkaido, northern Japan by Natsu Sugama and Ami Kawahata.
Phylum Echinodermata
Patiria pectinifera (Müller and Troschel, 1842): Cytochrome c oxidase submit I partial sequence of Patiria pectinifera (Echinodermata: Asterinidae) by Daiki Wakita and Takashige Funatsu.
Henricia nipponica Uchida, 1928: A sea anemone sequence from a sea star—28S rDNA partial sequence from Henricia nipponica (Echinodermata: Asteroidea) by Yuko Sugita and Yuko Nakazawa.
Luidia quinaria Von Martens, 1865: An attempt to extract DNA from the spiny sand seastar Luidia quinaria (Echinodermata: Asteroidea) collected in Oshoro Bay, Hokkaido, northern Japan by Yasushi Isozaki and Naho Yagi.