Report of Systematic Zoology Lab Practicum, June, 2012

Observation of a sylliid polychaete (Annelida: Sylliidae)

Takahiro Kato and Hiroki Ebina

Division of Biology, Department of Biological Sciences, School of Science, Hokkaido University, Sapporo 060-0810, Japan

Material and Methods
A sylliid polychaete was obtained intertidally in Oshoro Bay, Hokkaido, Japan, about 43° 21′ N, 140° 85′ E on 4 June 2012 by T. Kato and H. Ebina, photographed by H. Kajihara, and fixed in 99% EtOH. DNA was extracted from the entire specimen.
      PCR amplification of mitochondrial cytochrome c oxidase subunit I gene (COI) using LCO1490 (5′-GGTCAACAAATCATAAAGATATTGG-3′) and HCO2198 (5′-TAAACTTCAGGGTGACCAAAAAATCA-3′) (Folmer et al. 1994) was unsuccessful, probably due to a failure in DNA extraction.


Taxonomy
Phylum Annelida
Sylliidae gen. et sp. indet.
(Figs 1, 2)

Material examined: ICHU22100215 (non-extant, completely destroyed for DNA extraction).




Fig. 1. Sylliidae sp. (ICHU22100215), general shape.




Fig. 2. Sylliidae sp. (ICHU22100215), magnification of head.



Reference

Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294–299.