Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan
Introduction
In Systematic Zoology Lab Practicum 2014, 37 third-year undergraduate students in the Division of Biology, Department of Biological Sciences, School of Science, Hokkaido University, visited Oshoro Marine Biological Station on 19 May, 28 May, 2 June, and 9 June 2014 (Figs 1–4). They collected marine invertebrates among laminarian holdfasts and mussel beds. Each pair of students selected one invertebrate specimen, which was photographed in living state and fixed in 99% EtOH.
Fig. 1. Walking down a road on a hill in Oshoro town that leads to the marine station, 19 May 2014.
Fig. 2. Arriving at the marine station, 2 June 2014.
Fig. 3. Collecting specimens on the rocky shore in Oshoro Bay, 26 May 2014.
Fig. 4. Sorting the specimens in the laboratory of Oshoro Marine Biological Station, 9 June 2014.
In the Sapporo campus, the students extracted DNA, tried to PCR-amplify the mitochondrial cytochrome c oxidase subunit I gene (COI), and determined the sequence. In cases where COI amplification failed, they tired 16S and/or 28S rDNA instead.
Students' reports
Phylum Cnidaria
Actinia equina (Linnaes, 1758) Anthopleura pacifica Uchida, 1938: 28S rDNA partial sequence of Actinia equina (Cnidaria: Anthozoa: Actiniaria: Actiniidae) by Yui Hayashi and Marie Murakami.
Epiactis japonica (Verrill, 1869): 28S rRNA partial sequence of cf. Epiactis japonica (Cnidaria: Anthozoa) from Oshoro Bay, Hokkaido, Japan by Ryotaro Nouda an Nodoka Satoh.
Phylum Ctenophora
Beroe cucumis (Fabricius, 1780): Partial sequence of the nucleus-encoded 28S rRNA gene of the comb jelly Beroe cucumis (Ctenophora: Nuda) from Oshoro Bay, Hokkaido, northern Japan by Yoko Uchida and Takaaki Terada.
Phylum Nemertea
Quasitetrastemma nigrifrons (Coe, 1904): Cytochrome c oxidase subunit I gene partial sequence of Quasitetrastemma nigrifrons (Nemertea: Hoplonemerta: Monostilifera) from Oshoro Bay, Hokkaido, Japan by Chie Itoh and Kaho Sekiguchi.
Phylum Annelida
Phascolosoma scolops (Selenka and De Man, 1883): Nucleus-encoded 28S large subunit ribosomal RNA gene partial sequence of Phascolosoma scolops (Annelida: Sipuncula: Phascolosomatidae) from Oshoro Bay, Hokkaido, Japan by Haruka Takano and Kai Otsuka.
Phascolosoma agassizii Keferstein, 1866: Partial sequence of the mitochondrial cytochrome c oxidase subunit I gene of Phascolosoma agassizii (Annelida: Sipuncula) from Oshoro Bay, Hokkaido, Japan by Kaori Maehata.
Nereiphylla hera Kato and Mawatari, 1999: An attempt to sequence the mitochondrial cytochrome c oxidase subunit I, 16S rRNA, and the nucleus 28S rRNA genes of Nereiphylla hera (Annelida: Phyllodocidae) by Natsumi Takei and Osamu Horiguchi.
Arabella iricolor (Montagu, 1804): 28S large subunit ribosomal RNA gene partial sequence of Arabella iricolor (Annelida: Oenonidae) from Oshoro Bay, Hokkaido, Japan by Yu Yoshida and Shota Murakami.
Polynoidae sp.: Cytochrome c oxidase subunit I gene partial sequence of an unidentified polynoid polychaete (Annelida: Polynoidae) from Oshoro Bay on the Sea-of-Japan coast of Hokkaido, northern Japan by Yuuki Ohya and Masakazu Hoshino.
Marphysa sanguinea (Montagu, 1815): Partial sequence of the nucleus-encoded 28S rRNA gene from the polychaete Marphysa sanguinea (Annelida: Eunicidae) collected in Oshoro Bay on the Sea-of-Japan coast of Hokkaido, northern Japan by Tatsuhiko Komura and Kana Ishii.
Pseudopotamilla occelata Moore, 1905: 28S rDNA partial sequence of Pseudopotamilla occelata (Annelida: Sabellidae) from Oshoro Bay, Hokkaido, northern Japan by Akira Matuura and Haruka Yamaguchi.
Phylum Mollusca
Crassostrea gigas (Thunberg, 1793): An attempt to sequence the cytochrome c oxidase subunit I gene from the Japanese oyster Crassostrea gigas (Mollusca: Bivalvia) collected in Oshoro Bay, Hokkaido, Japan by Akane Asano and Nozomi Oshima.
Littorina brevicula (Philippi, 1844): Partial sequence of the nucleus-encoded 28S rRNA gene of Littorina brevicula (Gastropoda: Littorinimorpha: Littorinidae) from Oshoro, Hokkaido, Japan by Mana Nishikata and Rika Endo.
Vayssierea elegans (Baba, 1930): 16S ribosomal RNA gene partial sequence of Vayssierea elegans (Gastropoda: Nudibranchia) from Oshoro Bay, Hokkaido, Japan by Mieko Nakatuka and Akira Yamashita.
Phylum Arthropoda
Semibalanus cariosus (Pallas, 1788): Partial sequence of the mitochondrial cytochrome c oxidase subunit I gene of the prawn Trachysalambria curvirostris (Decapoda) from the barnacle Semibalanus cariosus (Cirripedia) by Yoshitake Sakai and Yudai Suzuki.
Cf. Ampithoe sp.: Mitochondrial cytichrome c oxidase subunit I gene partal sequence of an unidentified gammarid amphipod cf. Ampithoe sp. (Crustacea: Amphipoda) from Oshoro Bay, Hokkaido, Japan by Ayano Hata and Emi Kinoshita.
Gammaridea sp.: An attempt to sequence the mitochondrial cytochrome c oxidase subunit I gene of an unidentified gammaridean amphipod (Arthropoda: Malacostraca: Amphipoda) from Oshoro Bay, Hokkaido, Japan by Yuka Ogata and Konomi Toyooka.
cf. Ianiropsis longiantennata Thelemann, 1910: Partial sequence of the mitochondrial 16S rRNA gene determined from cf. Ianiropsis longiantennata (Crustacea: Isopoda: Asellota) from Oshoro Bay, Hokkaido, northern Japan by Mana Katoh and Motoko Kobayashi.
cf. Paridotea robusta (Nunomura, 1985): A partial sequence of the mitochondrion-encoded 16S rRNA gene of the valviferan isopod cf. Paridotea robusta (Crustacea: Idoteidae) from Oshoro Bay on the Japan Sea coast of Hokkaido, northern Japan by Ayako Hagiwara and Akiko Nagumo.