Report of Systematic Zoology Lab Practicum, Volume 6: e15; August, 2015

Observation of Lineus alborostratus (Nemertea: Pilidiophora) in Oshoro Bay, Hokkaid, Japan

Suda Kyoko and Matsumoto Yu

Division of Biology, Department of Biological Sciences, School of Science, Hokkaido University, Sapporo 060-0810, Japan

Material and Methods
A ribbon worm was obtained intertidally in Oshoro Bay, Hokkaido, Japan, about 43° 21′ N, 140° 85′ E on 4 June 2015 by K. Suda and Y. Matumoto, photographed by Shinri Tomioka, and fixed in 99% EtOH; the specimen was identified by Hiroshi Kajihara as Lineus alborostratus. Total DNA was extracted from a piece of body wall. PCR amplification was attempted for four gene markers, using the primer pairs LoboF1 (5′-KBTCHACAAAYCAYAARGAYATHGG-3′) and LoboR1 (5′-TGTTTYTTYGGWCAYCCWGARGTTTA-3′) (Lobo et al. 2013) for the mitochondrial cytochrome c oxidase subunit I gene, 16S ar-L (5′-CGCCTGTTTATCAAAAACAT-3′) and br-H (5′-CCGGTCTGAACTCAGATCACGT-3′) (Palumbi et al. 1991) for the mitochondrial 16S rRNA gene, H3aF (5′-ATGGCTCGTACCAAGCAGAC-3′) and H3aR (5′-ATATCCTTRGGCATRATRGTGAC-3′) (Colgan et al. 1998) for the nuclear histone H3 gene, and LSU5 (5′-ACCCGCTGAAYTTAAGCA-3′) and LSU3 (5′-TCCTGAGGGAAACTTCGG-3′) (Littlewood 1994) for the nuclear 28S rRNA gene. PCR products were visualized by electrophoresis in 1% agarose gel, but none of the four gene markers attempted were confirmed to be successfully amplified, probably due to a failure in DNA extraction.


Taxonomy
Phylum Nemertea
Class Pilidiophora
Order Heteronemertea
Family Lineidae
Lineus alborostratus Takakura, 1898
[Japanese name: Takakura-himomushi]
(Fig. 1)





Fig. 1. Lineus alborostratus (ICHU2130889), general shape.



References

Colgan, D. J., McLauchlan, A., Wilson, G. D. F., Livingston, S. P., Edgecombe, G. D., Macaranas, J., Cassis, G., and Gray, M. R. 1998. Histone H3 and U2 snRNA DNA sequences and arthropod molecular evolution. Austrarian Journal of Zoology. 46(5): 419–437.