Recent publications (1997-)


Fujimori, C. Ogiwara, K. Hagiwara, A. Rajapakse, S. Kimura, A. Takahashi, T. "Expression of cyclooxygenase-2 and prostaglandin receptor EP4b mRNA in the ovary of the medaka fish, Oryzias latipes: Possible involvement in ovulation" Molecular and Cellular Endocrinology 2010; In press

(58)Ogiwara, K. Ikeda, T. Takahashi, T. "A new in vitro ovulation model for medaka based on whole ovary culture." Zoolog. Sci. 2010; 27(9):762-7.

(57)Kato, Y. Ogiwara, K. Fujimori, C. Kimura, A. Takahashi, T. "Expression and localization of collagen type IV a1 chain in the medaka ovary." Cell Tissue Research 2010; 340(3):595-605.


(56)Takano, N. Kimura, A. Takahashi, T. "Two distinct localization patterns of testis-specific serine protease 1 (TESSP1) in the seminiferous tubules of the mouse testis." Zoolog. Sci. 2009; 26(4):294-300.


Horiguchi, M. Fujimori, C. Ogiwara, K. Takahashi, T. "Collagen type I α1 chain mRNA is expressed in the follicle cells of the medaka ovary." Zoolog. Sci. 2008; 25(9): 937-945.


Ogiwara, K., Takahashi, T. "Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological
tool for fusion-protein cleavage." Proc Natl Acad Sci USA 2007; 104(17): 7021-7026.

Rajapakse, S, Takahashi, T. "Expression and enzymatic characterization of recombinant human kallikrein 14." Zool Sci  2007; 24(8):774-780.

(52) Rajapakse, S, Yamano, N, Ogiwara, K, Hirata, K, Takahashi,S, Takahashi, T "Estrogen-dependent expression of the tissue kallikrein gene (Klk1) in the mouse uterus and its implications for endometrial tissue growth." Mol Repro Develop 2007; 74(8):1053-1063.


(51) Ogiwara, K and Takahashi, T "MODIFIED ENTEROPEPTIDASE POLYPEPTIDES."  Patent (2006)

(50) Rajapakse, S., Ogiwara, K., Yamano, N., Kimura, A., Hirata, K.,Takahashi, S., Takahashi, T. "Characterization of mouse tissue kallikrein 5."  Zool. Sci.  2006; 23(11): 963-968.


(49) Rajapakse, S., Ogiwara, K., Takano, N., Moriyama, A. and Takahashi, T. "Biochemical characterization of human kallikrein 8 and its possible involvement in the degradation of extracellular matrix proteins." FEBS Lett. 2005; 579(30): 6879-6884.

Matsui  H, Takano  N, Moriyama A and Takahashi  T "Single-chain tissue-type plasminogen activator is a substrate of mouse glandular kallikrein 24." Zoolog Sci. 2005; 22(10): 1105-1111.

Matsui  H, Takano  N, and Takahashi  T "Characterization of mouse glandular kallikrein 24 expressed in testicular Leydig cells." Int J Biochem Cell Biol 2005; 37(11): 2333-2343.

Ogiwara K, Takano N, Shinohara M, Murakami M, and Takahashi T "Gelatinase A and membrane-type matrix metalloproteinases 1 and 2 are responsible for follicle rupture during ovulation in the medaka." Proc Natl Acad Sci USA 2005; 102: 8442-8447.

(45) Takano  N, Matsui  H, and Takahashi  T "TESSP-1: A novel serine protease gene expressed in the spermatogonia and spermatocytes of adult mouse testes." Mol Reprod Develop 2005; 70(1):1-10.


(44) Takano  N, Matsui  H, and Takahashi  T "Granzyme N, a Novel Granzyme, Is Expressed in Spermatocytes and Spermatids of the Mouse Testis." Biol Reprod 2004; 71: 1785-1795.
(43) Ogiwara  K, Shinohara  M, and Takahashi  T "Expression of Proprotein Convertase 2 mRNA in the Ovarian Follicles of the Medaka, Oryzias latipes." Gene 2004; 337:79-89.

(42) Ogiwara  K, Shinohara  M, and Takahashi  T "Structure and Expression of Furin mRNA in the Ovary of Medaka, Oryzias latipes."  J Exp Zool 2004; 301A(5): 449-59.

(41) Patent 高橋孝行 "2本鎖組織プラスミノーゲンアクチベーターの製造法"  特許出願番号 特願平2004-99245号 (2004)

(40) Patent 高橋孝行、荻原克益 "魚類由来のエンテロペプチダーゼ"  特許出願番号 特願平2004-66632号 (2004)

(39) Ohnishi  J, Yokota  J, Rajapakse  R.G.S.C, Ohnishi  E, Kudo  T, Wada  S, and Takahashi  T "Activity of an Enzyme Converting Single-chain Tissue-type Plasminogen Activator to the Two-chain Form in Preovulatory Human Follicular Fluid."  Mol Reprod Develop 2004; 67: 178-185


(38) Ohkura R, Kimura  A, Kihara T, Ogiwara  K, and Takahashi  T "Expression of Bradykinin B2 Receptor in the Mouse Ovary. " Zool Sci 2003; 20: 847-854


(37) Kimura A, Matsui H, Takahashi T "Expression and localization of proryl oligopeptidase in mouse testis and its possible involvement in sperm motility" Zool Sci 2002; 19: 93-102

(36) Matsui H, Takahashi T "The presence of angiotensin-converting enzyme in follicular fluids of porcine ovaries and its possible involvement in the intrafollicular breakdown of bradykinin" Mol Reprod Dev 2002; 62 (1): 99-105

(35) Ogiwara K, Matsui H, Kimura A, Takahashi T "Molecular cloning and partial characterization of medaka fish stromelysin-3 and its restricted expression in the oocytes of small growing follicles of the ovary."Mol Reprod Dev 2002; 61: 21-31 


(34) Matsushima M, Yahagi N, Ichinose M, Miki K, Omata M, Inoue H, Takahashi T, Takahashi K "Inhibition of enteropeptidase by antitrypsin drugs"Biochemical Research 2001; 22 (5): 257-260

(33) Matsui H, Takahashi T "Molecular and biochemical characterrization of gladular kallikreins expressed in the adult mouse testis." Perspective in Comparative Endocrinology , pp. 1143-1146, Monduzzi Editore, Italy (2001)

(32) Kimura A, Ohkura R, Kihara T, Takahashi T "Bradykinin B2 receptor expression in the porcine ovary." Perspective in Comparative Endocrinology, pp. 1131-1134, Monduzzi Editore, Italy (2001)

(31) Ogiwara K, Shinohara M, Kimura A, Matsui H, Ohkura R, Takahashi T "Expression of matrix metalloproteinase in the ovary of the medaka fish Oryzias Latepes. " Perspective in Comparative Endocrinology, pp. 1167-1170, Monduzzi Editore, Italy (2001)

(30) Kimura A, Kihara T, Ohkura R, Ogiwara K, Takahashi T "Localization of B2 receptor in the follicles of porcine ovary and increased expression of matrix metalloproteinase-3 and -20 in cultured granulosa cells by bradykinin treatment." Biol Reprod 2001; 65: 1462-1470

(29) Matsui H, Takahashi T "Mouse testicular Leydig cells express mKlk21,a tissue kallikrein that cleaves fibronectin and insulin-like growth factor-binding protein-3." Endocrinology 2001; 142: 4918-4929

(28) Ohnishi J, Ohnishi E, Jin M, Hirano W, Nakane D, Matsui H, Kimura A, Sawa H, Nakayama K, Shibuya H, Nagashima K, Takahashi T "Cloning and characterization of a rat ortholog of MMP-23 (matrix metalloproteinase-23), a unique type of metalloproteinase and conditional switching of its expression during the ovarian follicular development." Mol Endocrinol 2001; 15:747-764

(27) Kimura A, Shinohara M, Ohkura R, Takahashi T "Expression and localization of transcripts of MT5-MMP and its related MMP in the ovary of the medaka fish Oryzias latipes." Biochim Biophys Acta 2001; 1518: 115-123


(26) Matsui H, Moriyama A, Takahashi T  "Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity." Eur J Biochem 2000;267:6858-6865

(25) Matsui H, Ogiwara K, Ohkura R, Yamashita M, Takahashi T "Expression of gelatinases A and B in the ovary of the medaka fish Oryzias latipes." Eur J Biochem 2000;267:4658-4668

(24) Yonezawa S, Kimura A, Koshiba S, Masaki S, Ono T, Hanai A, Sonta S, Kageyama T, Takahashi T, Moriyama A "Mouse myosin X: molecular architecture and tissue expression as revealed by northern blot and in situ hybridization analyses." Biochem Biophys Res Commun 2000;271:526-533

(23) Kimura A, Kihara T, Okimura H, Hamabata T, Ohnishi J, Moriyama A, Takahashi K, Takahashi T "Identification of porcine follipsin as plasma kallikrein and its possible involvement in the production of bradykinin within the follicles of porcine ovary." Mol Reprod Dev 2000;57:79-87

(22) Kimura A, Kakinuma K, Yonezawa S, Takahashi T "Expression of b-amyloid precursor protein in the porcine ovary." Zool Sci 2000;17:769-777

(21) Matsui H, Kimura A, Yamashiki N, Moriyama A, Kaya M, Yoshida I, Takagi N, Takahashi T "Molecular and Biochemical Characterization of a Serine Proteinase Predominantly Expressed in the Medulla Oblongata and Cerebellar White Matter of Mouse Brain." J Biol Chem 2000;275:11050-11057

(20) Kihara T, Kimura A, Moriyama A, Ohkubo I, Takahashi T "Identification of Components of the Intrafollicular Bradykinin-Producing System in the Porcine Ovary." Biol Reprod 2000;62:1160-1167

(19) Hirano W, Kimura A, Takahashi T "Presence of two distinct transcripts for matrilysin in porcine ovary." Zool Sci 2000;17:445-452

(18) Kimura A, Takahashi T "cDNA cloning of rat prolyl oligopeptidase and its expression in the ovary during the estrous cycle." J Exp Zool 2000;286:656-665


(17) Kimura A, Yoshida I, Takagi N, Takahashi T "Structure and localization of the mouse prolyl oligopeptidase gene." J Biol Chem 1999;274:24047-24053

(16) Matsushima M, Ichinose M, Yahagi N, Tsukada-Kato S, Miki K, Omata M, Kim YT, Ito H, Takahashi T, Sakurai Y, Tsuchiya Y, Athauda SB, Inoue H, Takahashi K "Purification and further characterization of enteropeptidase from porcine duodenum." J Biochem (Tokyo) 1999;125:947-951

(15) Murata M, Ohnishi J, Kudo T, Wada S, Yoshida H, Fujimoto S, Takahashi T "Proteolytic Activation of Single-chain Tissue-type Plasminogen Activator by Protease/a2-Macroglobulin Complex Isolated from Human Ovarian Follicular Fluid. " Zool Sci 1999;16: 285-290


(14) Kohyama K, Ohnishi J, Murata M, Takahashi T "Presence of follipsin complexed with alpha 2-macroglobulin in porcine ovarian follicular fluid." J Exp Zool 1998;280:57-64

(13) Kimura A, Ohnishi J, Okimura H, Hamabata T, Takahashi T "Localization of prolyl endopeptidase mRNA in small growing follicles of porcine ovary." Mol Reprod Dev 1998;50:121-127

(12) Takahashi T, Matsui H, Kihara T, Kimura A, Ohnishi J "Identification and partial characterization of a matallopeptidase from porcine ovaries. " J Exp Zool 1998;281:574-81

(11) Matsui H, Ohnishi J, Takahashi T "Proteolytic activation of tissue- type plasminogen activator by the culture media of mouse cancer cells. " Zool Sci 1998;15: 499-505

(10) Patent 高橋孝行、大西淳之"新規なメタロプロテアーゼ及びそれをコードするDNA" 特許出願番号 特願平10-1908868号(1998)

(9) Patent 大西淳之、高橋孝行"新規なメタロプロテアーゼ及びそれをコードするDNA" 特許出願番号 特願平10-1908869号(1998)


(8) Kihara T, Ohnishi J, Kohyama K, Moriyama A, Takahashi T "Identification and activation of profollipsin, a latent precursor form of porcine follipsin ." Eur J Biochem 1997;245:392-397

(7) Ohnishi J, Murata M, Kohyama K, Yoshida H, Wada S, Makinoda S, Fujimoto S, Takahashi T "Follicular fluid from human ovaries contains plasma kallikrein in free and in complex with a2-macroglobulin. " Biomedical Res 1997;18:161-170

(6) Kudo T, Ohnishi J, Murata M, Wada S, Yoshida H, Fujimoto S, Takahashi T "An isoform of plasma kallikrein occurring in the follicular fluid of human ovaries." Biomedical Res 1997;18:369-374

(5) Kim HJ, Tamanoue Y, Jeohn GH, Iwamatsu A, Yokota A, Kim YT, Takahashi T, Takahashi K "Purification and characterization of an extracellular metalloprotease from Psudomonas fluorescens." J Biochem 1997;121:82-88

(4) Matsuzaki H, Jeohn GH, Iwamatsu A, Tamanoue Y, Kim YT, Takahashi T, Takahashi K "Evidence for the identity of rat liver microsomal membrane-bound serine proteinase with hepsin." Protein Peptide Lett 1997;4:55-62

(3) Takahsahi T, Ohnishi J, Kihara T, Kimura A "Proteinases involved in follicle rupture during ovulation." Advances in Comparative Endocrinology 1997;1463-1468

(2) Kihara T, Kimura A, Ohnishi J, Takahashi T "A latent precursor form of follipsin and its activating enzyme." Advances in Comparative Endocrinology 1997;1545-1548

(1) Kimura A, Ohnishi J, Takahashi T "Analysis of prolyl endopeptidase activity and expression during follicular maturation in porcine ovary." Advances in Comparative Endocrinology 1997;1571-1575

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