Gallery

Lab equipments

We foucus on functions of several ovulation-related proteins. In constract to obtain enough amount of proteins (for example, enzymes) from animal tissue, production of recombinant proteins  is effeicient way for activity analyse. First of all, we preprae E. coli or mammalian cells expressing some recombinant proteins. Then, we purified them. Here, we introduce two equipments for purificartion of recombinant proteins.

 (left) HPLC (High performance liquid chromatography) :equipment to purify proteins by  affinity chromatography.      

(Right) Furaction collector: equipment to collect proteins purified by HPLC.         

 

 

  
                           

Second, we explore  functions of purified protein. Many kinds of factors are required for ovulation, for example, hormones, transcriptional factors and proteases. As an example, we introduce the equipment to investigate activities of proteases. We preprae labeled substrates and mix them with prified proteins. By using the equipment (as shown below) , we can detect the chemiluminecent afer enzymatic cleavage of labeled substrates by purified proteins.

 



                          

 


                    





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