Plants have a unique form of intercellular communication, as plant cells have symplastic connections via plasmodesmata. The symplastic passage of RNAs and proteins affects the cell differentiation state and helps transmit external environment signals. We have recently visualized this type of intercellular communication and have successfully developed an experimental system for quantitative analysis (see diagram). By using this system and pairing live cell imaging with mathematical analysis, we plan to uncover the molecular mechanisms of intercellular communication mediated by the plasmodesmata, and shed light on the unique information transmission method of plants.